I am proud to announce that an article, on which I have been lucky enough to collaborate, has now been published! As I mentioned in my short post about my thesis, I have been working as a research assistant at the department of immunology at my university in Debrecen, Hungary. It’s been an ongoing project for 4 years, which has now come to an end with a publication in the Journal: ‘Melanoma Research’!!!! And I am stoked! It was published a week ago, but I was not made aware of it before today (when I entered my own name in the search field on pubmed). I am still under restrictions as to what I can tell you about it, but now you can read it yourself from the online version of the journal HERE !
The Abstract reads: In the last three decades, the incidence of melanoma has increased worldwide and no effective treatment modalities have been developed yet. All-trans retinoic acid (ATRA) and polyinosinic:polycytidylic acid (polyI:C) are strong inducers of toll-like receptor 3 (TLR3) and MDA5 expression, and polyI:C-induced TLR3 and MDA5 signaling specifically causes cell death in melanoma cells in vitro. We addressed the question of whether ATRA pretreatment could enhance the efficacy of polyI:C and, if so, would ATRA have any additional effects on this process. We found that the combined treatment of human melanoma cells with ATRA and polyI:C strongly increased the expression of TLR3 and MDA5 in both WM35 and WM983A cells associated with significantly higher mRNA and secreted levels of interferon β (IFNβ), CXCL1, CXCL8/IL-8, CXCL9, and CXCL10 than cells treated with either ATRA or polyI:C. Silencing of MDA5 by siRNA moderately affected IFNβ secretion, whereas TLR3 knockdown interfered with both CXCL chemokine and IFNβ production. Furthermore, the supernatants of ATRA+polyI:C-activated cultures increased the migration of both human monocyte-derived macrophages and CD1a dendritic cells significantly as compared with the supernatants of cells treated with either ATRA or polyI:C, and this effect occurred in a TLR3-dependent manner. In conclusion, consecutive treatment with ATRA and polyI:C results in strong, TLR3/MDA5-mediated chemokine and IFN responses in cultured human melanoma cells, which triggers a functional migratory response in professional antigen-presenting cells. This novel mode of concomitant activation may represent a more efficient treatment option for future melanoma therapy.
*ATRA: all-trans-retinoic-acid. A derivative of vitamin A. It seems the effects of vitamin A go beyond those of anti-aging, and acne- curing in the skin. Check out my post about the anti-aging and anti-acne effects of Retinoic Acid, HERE.
This day has started on a rather awesome note. I’m so happy I don’t even mind unpacking all my baggage!
I’ll be back later today with an answer to a ROKer question, so stay tuned for that!
Have a lovely day!